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- 详细信息
- 文献和实验
- 技术资料
- 形态:
液体
- 保存条件:
-15°C to -25°C/1 year(Do not lower than -25°C)
- 保质期:
1年
- 库存:
100
- 供应商:
ImmunoWay Biotechnology Company
- 宿主:
Rabbit
- 应用范围:
WB,IHC,IF,IP,ELISA
- 规格:
40μL;100μL;200μL
兔重组基因工程抗体 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],
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文献和实验upon the dual pulldown to incorporate a third pulldown which is an iteration of the ChIP and is a pulldown for H3K27me3+ (Figure 1b). The third assay described here is the biotin-RNA pulldown of a low-copy RNA that spans the siRNA targeted promoter region
Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides
should also be included. In Ringrose et al ., (2004) I used histone H3 peptides spanning residues 1-13 (for lysine 4 and lysine 9 modifications) or 21-33 (for lysine 27 modifications). Stock solutions of 0.5mM peptide in PBS can be prepared and kept at -20°C
upon the dual pulldown to incorporate a third pulldown which is an iteration of the ChIP and is a pulldown for H3K27me3+ (Figure 1b). The third assay described here is the biotin-RNA pulldown of a low-copy RNA that spans the siRNA targeted promoter region
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