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Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (SSⅣ M-MLV)是从克隆有经基因改造的Moloney Murine Leukemia Virus reverse transcriptase基因载体在大肠杆菌中经诱导表达分离纯化而来。该酶可在37-65℃用于cDNA第一链的合成,提供持续的特异性的扩增,高效的生产cDNA,它可反转录比其它酶更长的片段,cDNA片段的长度在100bp~18kb范围之间。其最佳反应温度是50-55℃,具有灵敏度高、特异性强、聚合活性高和半衰期长的特点。该酶是M-MLV和RNase抑制剂的混合酶,可减少加样步骤,降低污染风险。
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文献和实验The recovery of vectors that are suitable for an in vivo gene delivery has been a recurrent theme in gene therapy research over the last decade. Several challenging hurdles need to be overcome to reach such a goal
M-MLV第一链cDNA合成试剂盒-技术手册一、 试剂盒组成 Components SK2027 SK2028 5×M-MLV Reaction Buffer 100µl
Detection, Quantification, and Characterisation of HIV/SIV
Selected techniques for the detection, quantification, and characterisation of HIV1, HIV2, and SIV, as applied to diagnostic and research purposes, are described. Representative nucleic acid testing protocols including nested PCR, RT
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