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- 文献和实验
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- 保存条件:
详询
- 库存:
99
- 供应商:
广州市左克生物
- 规格:
100μg
【抗体内化检测试剂盒】
货号:001-106-103
种属:Human
标签/类型:Nano-secondary antibody-Drug conjugates
临界点⽣物为抗体内化检测提供了与MMAE、MMAF、DXD或PBD等相关的新⼀代纳⽶⼆级抗体-药物偶联物。在基于细胞 的细胞毒性试验中使⽤纳⽶⼆次抗体-药物偶联物(Nano-Secondary ADC)是⼀种快速且经济的替代⽅法 ,可⽤于预筛选 抗肿瘤细胞的候选ADC单克隆抗体。Nano-Secondary ADC在缺乏⼀抗的情况下对细胞具有⾼度特异性和最⼩毒性 ,当偶 联⼆抗存在时 ,⼀抗活性⽆明显变化。
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文献和实验-20 in 50 mM Na-phosphate buffer, pH 7.3. Goat-anti-mouse antibodies labeled with AlexaFluor-488 were from Invitrogen, Inc. (dye/protein ratio 7). Microscope glass slides, 3 × 1 in., and 1 mm thick, were from VWR. Water was purified by Milli-Q
, dry 5. block half of the plate (with platelets) by PBS-BSA 1% and coat another half by glycine (x1) - BSA for 1 hour at 37ѓC 6. wash 3 times in PBS-tween 20, dry 7. incubated at 37ѓc for 1 hour with a 1:1000 dilution of rat IgG2a anti-mouse kappa
E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
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