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- 2025年07月21日
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- 库存：
1000
- 供应商：
北京博蕾德生物科技有限公司
- 规格：
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文献和实验Generation of Model Cell Lines Expressing Recombinant G-Protein-Coupled Receptors
The molecular cloning of the cDNA sequences encoding most G-protein-coupled receptors, including those from humans, allows their study in a variety of recombinant systems. In this respect, transfected mammalian cell lines constitute
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
have a promoter upstream of the MCS such that the insert gets transcribed. If the promoter is mammalian (e.g., the CMV promoter), the transcription will take place only in mammalian systems (like mammalian cell culture), but not in the bacteria. Plasmids
Recombinant expression and SDS
Gels 15% 4% 30% Acrylamide 5 ml 0.65 ml 4X Tris-HCl, pH 8.8 2.5 ml 4X Tris-HCl pH 6.8 1.25ml ddH2O 2.5 ml 3.0 ml 10% Ammonium Persulfate 33 ml 50 ml TEMED 7 ml 7 ml SDS electrophoresis buffer, 5× 15.1 g
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