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- Cayman
- 32163-100 μg
- 2025年07月21日
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文献和实验Monoclonal Antibody Production Protocol
1 - Substrate = ABTS - 100X (from Zymed 00-2001)1 - HRP buffer: 100mM Na Citrate pH 4.2( 490 mg citric acid + 720 mg Na citrate dehydrate + 50 ml H2O, pH 4.2 ). - Hydrogen peroxide 30% ( 1000X ) Protocol: ABSORBTION OF ANTIGEN Dilute Ag to 10 ug/ml in PBS
Polyclonal and Monoclonal Antibodies
). Enzyme linked antibody = Horseradish peroxidase 1 Substrate = ABTS - 100X (from Zymed 00-2001)1 HRP buffer: 100mM Na Citrate pH 4.2( 490 mg citric acid + 720 mg Na citrate dihydrate + 50 ml H2O, pH 4.2 ). Hydrogen peroxide 30
4. Remove solution and rinse ovaries with PBS 3 times, then wash 3 times for 10 minutes each 5. It may help antibody penetration to dissect apart the egg chambers 6. Incubate ovaries in PBT for 10 minutes 7. Add monoclonal supernatant
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