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for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
FITC荧光二抗?――EarthOx新型DyLight 488绿色荧光
AffiniPure Goat Anti-Rabbit IgG(H+L) EarthOx E032220 ¥200/100μl ¥800/500μl 详情 DyLight 488 AffiniPure Rabbit Anti-Goat IgG(H+L) EarthOx E032230 ¥200/100μl
Anti-GFP/BrdU In Situ Immunostaining
by adding 1 mL PBS, 3' each. Add 0.3 mL rabbit anti-GFP (1:200 v/v, plus 1% goat serum in PBS) and incubate 1 hr. at room temp. Wash 3x in PBS. Post-fix in 1 mL 4% paraformaldehyde x15', room temp. Wash 3x in PBS. Denature DNA with 1 mL
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