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- 2025年07月21日
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文献和实验Identification of Novel and Known miRNAs in Deep‐Sequencing Data with miRDeep2
it possible to reliably identify miRNAs with computational methods. This unit documents how to use the miRDeep2 software package to identify novel and known microRNAs in small RNA deep?sequencing data. Moreover, the usage of miRDeep2 to profile miRNA
In Vivo Imaging of Far-red Fluorescent Proteins after DNA Electrotransfer to Muscle Tissue
DNA electrotransfer to muscle tissue yields long-term, high levels of gene expression; showing great promise for future gene therapy. We want to characterize the novel far-red fluorescent protein Katushka as a marker for gene
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
Inc.) or QiaFilter™ (Qiagen Inc.) plate positioned over a 96 deep-well collection block inside a vacuum manifold. Transfer 600 ul at a time, collecting the top half (with flocculent ppt.) first. Vacuum filter using approximately 300-500 mm Hg until filtration
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