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文献和实验Generation and Selection of Rabbit Antibody Libraries by Phage Display
The rabbit antibody repertoire is an exceptional source for both polyclonal antibodies (pAbs) and monoclonal antibodies (mAbs) that combine high specificity with high avidity and affinity, respectively. In contrast to rabbit pAbs
attractive not only for studying various aspect of BCR signaling but also for the generation of human monoclonal antibody and the analysis of the human B cell repertoire. This chapter describes an efficient method to immortalize and clone human B cells
Production of a Human Antibody Library in the Phage-Display Vector pSEX81
(1 ,2 ). A promising alternative 1s the production of human recombinant antibodies (3 ). Recombinant DNA technology has made it possible to clone human antibody genes in vectors and to generate antibody expression libraries (4–7). One approach has been to amplify
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