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- 2025年07月21日
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文献和实验Monoclonal Antibody Production
(note the removal of the HT)in a T25 flask (one for each positive clone)for later use (7) When the cells are ready for expansion out of the 24-well plate,resuspend them and pipet to the T25 flasks with feeder cells.Again leave a little bit in the 24
Monoclonal Antibody Production
Harvest more feeder cells for your next expansion. Put 0.5 mL f feeder cells in DMEM/20 FCS (note the removal of the HT) in a T25 flask (one for each positive clone) for later use When the cells are ready for expansion out of the 24-well plate
Human Monoclonal Antibodies: The Residual Challenge of Antibody Immunogenicity
One of the major reasons for seeking human monoclonal antibodies has been to eliminate immunogenicity seen with rodent antibodies. Thus far, there has yet been no approach which absolutely abolishes that risk for cell-binding antibodies
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