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文献和实验Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides
to chromatin, by its histone tail interaction or by other interactions, then you would not expect to see loss of binding upon peptide competition. A good positive control for the assay is to use H3K9 dimethylated peptide to compete HP1 from the pericentric
Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization
control) relative to the level of the signal of interest (ChIP sample). This hampers distinguishing a ''true signal'' from the background signal. Therefore, we recommend setting up ChIP using an antibody against an invariant domain of histone H3 [19,20
," made up of fragments of up to seven nucleosomes in length, is incubated with an antiserum directed against the histone modification of interest. The antibody-bound fraction is separated from the unbound fraction and, after extraction of genomic DNA from the bound
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