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- 2025年07月21日
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文献和实验Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization
: the untranslated leader (UTR) and the second exon (exon 2; Figure 5a). We observed positive signals for both the actin UTR (Figure 5b) and exon 2 (Figure 5c) when using antibodies against H3K4me2, H3K9ac/K14ac (H3ac), H4ac, but no signals above background
Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides
to chromatin, by its histone tail interaction or by other interactions, then you would not expect to see loss of binding upon peptide competition. A good positive control for the assay is to use H3K9 dimethylated peptide to compete HP1 from the pericentric
that was an interspecific hybrid (H) between Mus musculus domesticus (D, paternal genome) and Mus spretus (S, maternal genome). The native chromatin was then immunoprecipitated with rabbit polyclonal antisera to acetylation at lysine 9 of H3 (H3K9Ac) and to dimethylation
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