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文献和实验The production of monoclonal antibody by hybridoma
The production of monoclonal antibody by hybridoma fusion: Immortalization of sensitized B lymphocytes from immune mice. Overview
The optimal exposure time will depend on the amount of 32 P used and the amount of kinase activity. Generally a few hours should be fine. SOLUTIONS Histone Wash Buffer 25 mM Tris HCl, pH7.5 70 mM NaCl 10 mM MgCl2 1 mM DTT
Polyclonal and Monoclonal Antibodies
later again (2 + 2 week cycle). Monoclonal Antibody If titre is positive do one of the following 2 weeks later: I - Boost tail vein with 20 ug- 50 ug of Antigen in PBS and proceed with fusion on day 4. OR II - Boost
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