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- Cayman
- 32180-100 μg
- 2025年07月21日
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文献和实验monoclonal antibody production fusion
1 ml of pre-warmed (37ѓC) 50% PEG 1500 (polyethylene glycol, Roche, Germany) continously stirring the cells with the pipette tip (this procedure lasts for a period over 1 min) add 1 ml of pre-warmed to 37ѓC IMDM continously stirring
Monoclonal Antibody Production
(note the removal of the HT)in a T25 flask (one for each positive clone)for later use (7) When the cells are ready for expansion out of the 24-well plate,resuspend them and pipet to the T25 flasks with feeder cells.Again leave a little bit in the 24
Monoclonal Antibody Production Protocol
of debris, rounded and refractive under phase contrast, and that they are harvested in log or late log phase growth (between 3.5 and 9 x105 cells/ml). Thaw cells 7 days before scheduled fusion. Myeloma do not grow well after being in culture
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