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- 32344-100 μg
- 2025年07月21日
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文献和实验Chromatin Immunoprecipitation (ChIPs) Cloning Protocol (Farnham Lab)
). Day 2 14. If you are not using rabbit polyclonal antibodies (ie. monoclonal antibodies, ect), add 1 ug of an appropriate secondary antibody (rabbit) and incubate for an additional 1 hour. 15. Add
population is termed monoclonal . It recognizes a single epitope on the antigenic protein. Antibody producing B lymphocytes can be isolated from the spleen or from lymph nodes. However, they have a finite life span in culture, i.e
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
). 2. Dishevelled (Dvl)-2 primary antibody: clone 10B5 (sc-8026;Santa Cruz Biotechnology). 3. Dvl-3 primary antibody: clone 4D3 (sc-8027; Santa CruzBiotechnology). 4. Secondary antibody to use with mouse monoclonal primaryantibodies: sheep anti-mouse IgG-HRP
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