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- 2025年07月21日
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文献和实验Monoclonal Antibody Lead Characterization: In Vitro and In Vivo Methods
This chapter describes in vitro and in vivo methods to characterize a lead monoclonal antibody candidate in the drug discovery setting. Approaches to characterize monoclonal antibody specificity, heavy and light chain composition
Phage-Display Libraries of Murine and Human Antibody Fab Fragments
This chapter describes efficient procedures for construction, expression, and screening of comprehensive libraries of murine or human antibody Fab fragments displayed on the surface of filamentous phage. Phagemid vectors are used for placing
Expression Cloning of Human B Cell Immunoglobulins
from the flow cytometric isolation of single human B cells, to the RT-PCR-based amplification of the expressed Igh , Igκ , and Igλ chain genes, and Ig gene expression vector cloning for the in vitro production of monoclonal antibodies. The strategy may be used
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