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文献和实验Production of Chimeric Heavy-Chain Antibodies
of fragment crystallizable (Fc), which is known to be crucial for a long serum half-life through binding to FcRn and antibody-mediated cell killing through binding to Fcγ receptors and complement. Single-domain antibodies (sdAbs) derived from camelid heavy
Monoclonal Antibody Lead Characterization: In Vitro and In Vivo Methods
This chapter describes in vitro and in vivo methods to characterize a lead monoclonal antibody candidate in the drug discovery setting. Approaches to characterize monoclonal antibody specificity, heavy and light chain composition
Expression and Isolation of Recombinant Antibody Fragments in E. coli
antibodies (MAbs), functional derivatives (antibody fragments) carrying antigen (Ag)-binding activities, and comprising at minimum the antibody variable heavy- and light-chain domains, can be readily produced in bacterial cells (1 ). The range of antibody
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