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文献和实验each of seven different α and β subunits arranged into four stacked rings (α7 β7 β7 α7 ). Separation by two-dimensional (2D) gel electrophoresis of the human erythrocytes 20S proteasome subunits and mass spectrometry (MS) identification of all the observed spots reveal
Co-Expression of human DNA primase p49-p58 subunits
.Note: You don't have to shake (the Fanning lab simply transfers their flasks to a RT H2O bath),but I found that it does improve expression levels. 6.Harvest by centrifugation,5000 rpm,10 min.,4℃.Freeze pellets at -80℃. Purification of p49-p58 human
Preparation of Native and Recombinant Light-Harvesting Chlorophyll-a/b Complex
Procedures to isolate native light-harvesting chlorophyll-a/b complex (LHCIIb) and to reconstitute recombinant LHCIIb are described. Separation of trimeric from monomeric forms and free pigment by sucrose density-gradient ultracentrifugation
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