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VE-Cadherin Anti-Cad 3 Polyclo

nal Antibody
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  • ¥1
  • Cayman
  • 32754-100 μg
  • 2025年07月21日
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    • 详细信息
    • 文献和实验
    • 技术资料
    宿主:兔子;应用:WB、IF。Host: Rabbit; Applications: WB, IF.分子式分子量

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    相关实验
    • Protocol for anti-HA antibody Western Blotting

      ) 5) Incubate in primary antibody solution: I use PBS with 0.1% TWEEN-20 plus 5% BSA as the basic solution, but you can use TBS too. I dilute the HA antibody 1:5000, just to be sure you will see a signal. I haven't EVER seen appreciable background. I've

    • Protocol for anti-HA antibody Western Blotting

      it around for 5-10 seconds, and pour it off: this method was worked out in the Yamamoto lab eons ago). 7) Incubate with secondary antibody (anti-mouse) in 5% BSA/PBS/Tween as you would ordinarily. I usually go for 2 hours @ room temp. 8) 12-15 quick washes

    • Anti-HA Immunocytology

      . A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.

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