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文献和实验Identifying Acetylated Proteins in Mitosis
the acetylation status of several mitotic proteins by anti-acetyl-Lysine immunoprecipitation with a monoclonal antibody followed by Western blot analyses of the proteins of interest. We further confirmed by a reciprocal immunoprecipitation with protein-specific
-phosphoserine的抗体。 摘抄： Anti-Phosphothreonine (MAb) - CryptateThe anti-phosphothreonine antibody is a monoclonal antibody (clone 42H4) produced at Cell Signaling Technology (reference 9386). It recognizes phosphorylated threonine residues in a manner
核蛋白基因组指纹技术Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured Cells
, pepstatin; each final concentration 2 µg/ml) Protein A/G agarose beads Antibody Dilution buffer (stable at room temperature; add PMSF and proteinase inhibitors and put on ice before use) 10 mM Tris-HCl (pH 8.0)
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