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文献和实验HPLC‐SEC Characterization of Membrane Protein‐Detergent Complexes
Figure 29.5.2 Data collection window for BSA fractionated in 0.5% (w/v) DDM on a TOSOH G40000SWxl column, 7.8 × 300 mm at 0.4 ml/min. The overlaid real time signals from the SLS (“LS11”‐90° detector), UV
100 mM MgCl 2 5 mM coenzyme A (see recipe ) Radiolabeled carboxylic acid substrate (e.g., 100 µM 80
Identification and Analysis of Multiprotein Complexes Through Chemical Crosslinking
. Each lane contains 10 µl of protein sample from the conjugation reaction. (B ) Protein complexes formed using two TFIIB mutants (R37C and V35C) with surface cysteine residues conjugated to 125 I‐PEAS as well as a control protein (WT, wild type
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