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文献和实验with a recombinant baculovirus that carries a DNA insert encoding for the entire extracellular domain, including the N-terminal signal sequence.
Expression of Extracellular N-Terminal Domain of NMDA Receptor in Mammalian Cells
consists of two functionally distinct regions: a large amino-terminal extracellular domain containing the putative agonist binding site and a hydrophobic core consisting of four transmembrane-spanning domains that participate in the formation of the ion
For recombinant expression of extracellular matrix (ECM) proteins or their individual domains, the use of transformed mammalian cells offers two major advantages. First, eukaryotic expression can be expected under optimum conditions
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