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文献和实验Evaluation of Histone-Modifying Enzymes in Stem Cell Populations
The histone demethylases are a relatively novel family of histone-modifying enzymes. Their gene expression suggests that each of the subfamily members may have a discrete role in cell function. The KDM5 family of H3K4 histone demethylases
In Vitro and In Vivo Assays for Studying Histone Ubiquitination and Deubiquitination
al., Nature 449:1068–1072, 2007) and H2B ubiquitination in transcription, H3 methylation, and DNA methylation (Zhang, Genes Dev 17:2733–2740, 2003; Sun and Allis, Nature 418:104–108, 2002; Sridhar et al., Nature 447:735–738, 2007
Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides
to chromatin, by its histone tail interaction or by other interactions, then you would not expect to see loss of binding upon peptide competition. A good positive control for the assay is to use H3K9 dimethylated peptide to compete HP1 from the pericentric
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