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文献和实验Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
Steve HahnProtein Dilution Buffer 5ml20 mM Tris pH7.9 100 microliters 1M Tris 7.9150 mM KCl 0.75 ml 1 M KCl1 mM DTT 50 microliters 0.1 M DTT10% glycerol 1 ml 50% glycerol50 micrograms/ml BSA 2.5 microliters 100 mg/ml BSA3.1 ml H2OOptional: add Brij
KSUD14-STS ASSAY FOR TAGGING Lr21
New D14 primers. Lr21L: CGC TTT TAC CGA GAT TGG TC Lr21R: TCT GGT ATC TCA CGA AGC CTT Lr21 : 669 bp lr21 -Fielder: 757 bp lr21 -Wichita: 774 bp Recipe (in 25 µl reaction
method on several common models of qPCR instruments, how to prepare the samples for the assay, and how to run and analyze the resulting data. The unit also describes a 96?well screen to determine optimal buffer conditions for protein stability
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