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文献和实验Microarray Approaches for Analysis of Cell Cycle Regulatory Genes
Cell cycle phase-specific regulation of transcription is a major mechanism for the regulation of progress through the eukaryotic cell cycle. Hundreds of genes are known to be cell cycle regulated, including histones, cyclins, transcription
/2 or total ERK1/2 MAP Kinases), then with horseradish peroxidase-conjugated second antibody and detected by enhanced chemiluminescence according to the manufacturer’s intructions. Molecular weights of proteins were estimated by using prestained SDS–PAGE
: H-6000 or H*2 flow autoanalyzer (Bayer Co) electronic cell counter model 2F Channelyser model 256 (Coulter Electronics) REFERENCES Bergmeier, W., Rackebrandt, K
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