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Protein A/G Coated Plate Immun

oprecipitation Kit
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  • ¥1
  • Cayman已认证
  • 601970-1 ea
  • 2025年07月21日
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    • 文献和实验
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    Cayman的Protein A/G包被板免疫沉淀(IP)试剂盒提供了一种便捷的方法,用于从细胞裂解液、血清、杂交瘤培养基以及重组蛋白或重组抗体培养基中捕获和浓缩目标蛋白,且采用96孔板格式。在从Protein A/G包被的96孔条板上洗脱捕获的蛋白后,可使用SDS-PAGE、Western blot和质谱分析等方法进行进一步分析。与传统的管式或树脂基免疫沉淀方法相比,该试剂盒具有以下优势:1.96孔板格式允许更高的通量,用户可以同时处理多达96个样本。2.洗涤时间大大缩短,因为可以通过多通道移液器在几秒钟内完成孔的洗涤,而传统的管式洗涤则需要几分钟。3.在洗涤或样本加载过程中不会意外转移树脂珠子。Cayman's Protein A/G Coated Plate Immunoprecipitation (IP) Kit provides a convenient method for the capture and concentration of target proteins from cell lysates, serum, and hybridoma culture media, as well as recombinant protein or recombinant antibody media, in a 96-well plate format. Following elution of the captured proteins from Protein A/G Coated 96-well strip plate, SDS-PAGE, Western blot, and mass spectrometry can be used for further analysis. This kit provides the following advantages over traditional tube- or resin-based IP methods: 1.The 96-well plate format allows for higher throughput by allowing the user to work with up to 96 samples 2.Much less time spent washing, as wells can be washed via multichannel pipette in seconds versus the minutes required for tube washes 3. No accidental transfer of resin beads between washes or during sample loading.分子式分子量

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    相关实验
    • Dynabeads® Co-Immunoprecipitation Kit

      , although the coupling reaction also works well with antibodies in storage buffers that include protein additives (e.g. BSA) and/or sodium azide (NaN3). This kit is not recommended for use with antibodies that have been stabilized in glycerol. 实验试剂  

    • AllPrep DNA/RNA 96 Kit

      A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96

    • Chromatin Immunoprecipitation (ChIP)

      to cross-link proteins to DNA irreversibly. The cross-linked chromatin was then either sonicated or cleaved with restriction enzymes to generate smaller DNA fragments, followed by immunoprecipitation with the desired antibodies. The precipitated protein

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