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文献和实验Chemiluminescent Detection of Senescence-Associated Galactosidase
Identifying molecules that serve as markers for cell aging is a goal that has been pursued by several groups. Senescence-associated β galactosidase (SA-βgal) staining is broadly used and very easily detected. β-gal is a lysosomal enzyme
Colorimetric Detection of Senescence-Associated Galactosidase
, we described the “senescence-associated β galactosidase” (SA-βgal) biomarker, which conveniently identifies individual senescent cells in vitro and in vivo. Here, we describe an updated protocol for the detection of cell senescence based on this widely used
Methods to Detect Biomarkers of Cellular Senescence: The Senescence-Associated -Galactosidase Assay
phenotype, a senescence associated β-galactosidase (SA-β-gal), which is detected by histochemical staining of cells using the artificial substrate X-gal. The presence of the SA-β-gal biomarker is independent of DNA synthesis and generally distinguishes
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