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文献和实验All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies
Brief protocol using Digest-All™ Kit is described below. The Digest-All™ is a flexible and standardized system for tissue digestion and contains trypsin, ficin, and pepsin proteolytic enzymes. The degree of digestion is based on a standardized 10 minute
A general protocol for staining cell for cytometry analysis
General Annexin V Staining Procedure Solutions 1.10X Binding Buffer (Cat. No. 66121A):0.1 M HEPES, pH 7.4; 1.4 M NaCl; 25 mM CaCl 2 . Dilute to 1× prior to use. 2. Propidium Iodide (PI). Prepare a 50 µg/ml stock solution of PI in 1× PBS buffer
Differential Staining of Live and Dead Embryos using Fluorescein Diacetate
for staining of all cells and not just embryos or oocytes . Also, ethididium bromide can be replaced with propidium iodide, DAPI or Hoescht 33342 Materials Ethidium Bromide (EtBr ; Fisher
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