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文献和实验HIS-tagged protein purification
John Mundy,Institute of Molecular Biology,Copenhagen,Denmark 1 liter cell prep 1)grow 20ml cells O/N37℃ dilute 50X into prewarmed LB,grow to 0.6 OD or about 1hr.Induce w/ 2mM IPTG (238mg/0.5l),grow 3hr,spin 6k GS3 10',freeze at 70℃ 2)resuspend
Antisense Inhibition of Protein Synthesis and Function: Rabbit Retinal Protein
When considering the use of antisense technology for in vivo apphcatron, whether for therapeutic development or for the creation of animal models for human diseases, a major problem is that most extracellular compartments are constantly
Methods for Generation and Analysis of Fluorescent Protein-Tagged Maize Lines
. To date, protein localization in this species has mostly been performed using immunolocalization with specific antibodies, when available, or by overexpression of fluorescent protein fusions. Localization of tagged proteins using native regulatory elements
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