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文献和实验Analysis of Fatty Acid Amide Hydrolase Activity in Plants
acid by a membrane enzyme known as the fatty acid amide hydrolase (FAAH). Thus, FAAH represents an important target to better understand the function of these lipid mediators in numerous cellular processes. FAAH has been extensively characterized
Assay of Membrane Transport of Long-Chain Fatty Acids by Adipocytes
that the contribution of the transport component to cellular FA uptake will depend on several factors, including the concentration of free or unbound FA, the cell type, and so on.
conjugation to a lipid domain (“Lip,” such as a fatty acid), thereby creating “CatLip”-conjugates. The cellular uptake of these conjugates is conveniently evaluated using a sensitive cellular assay system based on a splicing correction of a mutated luciferase
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