Caspase-1 Inhibitor Screening

Cayman的Caspase-1抑制剂筛选检测试剂盒提供了一个强大且易于使用的平台，用于识别人类caspase-1的新型抑制剂，caspase-1是炎症小体激活后的主要下游效应物。该检测使用caspase-1特异性荧光底物Ac-YVAD-AFC。Caspase-1裂解该底物产生游离AFC，可使用荧光板读数器在激发和发射波长分别为400和505nm时轻松定量。强效且可逆的caspase-1抑制剂Ac-YVAD-CHO被列为阳性对照。Cayman's Caspase-1 Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of human caspase-1, the major downstream effector following inflammasome activation. The assay uses a caspase-1-specific fluorogenic substrate, Ac-YVAD-AFC. Caspase-1 cleaves this substrate generating free AFC, which can be easily quantified using a fluorescence plate reader at excitation and emission wavelengths of 400 and 505 nm, respectively. The potent and reversible caspase-1 inhibitor Ac-YVAD-CHO is included as a positive control.分子式分子量