[13C3]-Dihydrotestosterone

5α－二氢睾酮5α－dihydrotestosterone

C 19 H 3 0O 2 。亦称 17 β－羟基 -5 α - 雄 [ 甾 ] 烷 -3- 酮、二氢睾酮、 5 α－ DHT 、或雄烷酮（ stanorone ）。是睾酮的双键在 5 α处被还原的甾醇。对附属腺（前列腺、精囊腺等），起着所谓的真的雄性激素的作用。在附属性腺的细胞中存在着形成该甾醇的 5 α - 氢化酶。该甾醇和受体蛋白质在细胞内结合成的复合体进入附属腺的细胞核中，促进新的蛋白质合成。显示出比睾酮稍强的雄性激素作用。 5 β - 体虽在肝脏、鸡冠等中以睾酮形态存在

Production and Purification of Histidine-Tagged Dihydrotestosterone-Bound Full-Length Human Androgen Receptor

Protein purification and characterization is required for a full understanding of structure-function relationships. Because proteins have complex structures and can be present at low concentrations, efficient purification protocols are needed

Isotopically Nonstationary 13C Metabolic Flux Analysis

13 C metabolic flux analysis (MFA) is a powerful approach for quantifying cell physiology based upon a combination of extracellular flux measurements and intracellular isotope labeling measurements. In this chapter, we present the method