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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
广州威佳科技有限公司
- CAS号:
352195-40-5
- 规格:
100mg
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文献和实验DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
temperature for 5 minutes. 9. Centrifuge at maximum speed ($13,000 x g) at 4°C for 10 minutes to precipitate Nucleic acid. Discord the supernatant. 10. Wash the nucleic acid pellet by adding 400 μL of 70% ethanol into the tube. Vortex the tube
?cyanoethylthio group is effected by treatment with 2,4,6?triisopropybenzenesulfonyl chloride and triethylamine to give a 6?(2,4,6?triisopropylbenzenesulfonic acid) ester intermediate. Reaction of this key intermediate with 3?mercaptoproprionitrile
33 g disodium EDTA 9.3 g 5.58 (The TBE should be fresh-if the boric acid precipitates, make a fresh stock--we find that a 6x solution lasts longer before precipitating
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