15-hydroxy Prostaglandin Dehyd

Cayman的15-PGDH抑制剂筛选检测试剂盒提供了一个强大且易于使用的平台，用于识别人类15-PGDH的新型抑制剂，15-PGDH是一种催化前列腺素氧化为15-酮代谢物且生物活性降低的酶。通过监测NAD+还原为NADH来测量15-PGDH活性。NAD+还原为NADH伴随着在340nm激发后445nm荧光的增加。强效且可逆的15-PGDH抑制剂ML-148检测试剂作为阳性对照，可抑制15-PGDH，IC50值为11nM。Cayman's 15-PGDH Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of human 15-PGDH, the enzyme that catalyzes the oxidation of prostaglandins to 15-keto metabolites with reduced biological activity. Measurement of 15-PGDH activity is carried out by monitoring the reduction of NAD+ to NADH. The reduction of NAD+ to NADH is accompanied by an increase in fluorescence at 445 nm following excitation at 340 nm. The potent and reversible 15-PGDH inhibitor ML-148 Assay Reagent is included as a positive control and inhibits 15-PGDH with an IC50 value of 11 nM.分子式分子量