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文献和实验Evaluating Modulators of “Regulator of G‐protein Signaling” (RGS) Proteins
Modulators with the Transcreener GDP Assay and a Rate‐Modified Gα Subunit Basic Protocol 2: Measuring Disruption of the RGS Domain/Gα Interaction by Förster Resonance Energy Transfer (FRET) Basic Protocol
Xu, X., Brzostowski, J.A., and Jin, T. 2006. Using quantitative fluorescence microscopy and FRET imaging to measure spatiotemporal signaling events in single living cells. Methods Mol. Biol. 346:281‐296.
, Hedgehog, and insulin pathways, as well as the activationof nuclear factor (NF)-kB-mediated gene transcription. These signaling pathways regulate gene transcription,cell cycle, apoptosis, inflammation, glucose metabolism, stem-cell renewal
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