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- Cayman
- 36551-100 mg
- 2025年07月20日
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文献和实验is the same for all species, there are some differences in handling cells. This protocol is developed and tested for E. coli . It should work the same way for other bacteria but that remains to be proven. Published protocols also exist for other bacterial species, including
Maxiprep of plasmid DNA from E. coli
worth of components for solution 2: 200μL 10% SDS, 100μL 4M NaOH, 1.7 mL autoclaved water 1.5mL Solution 3: 3M K+ , 5M acetate (3M potassium-acetate, 2M acetic acid -- glacial is 17M) 3.7mL isopropanol 1mL TE buffer 0.5mL 5M LiCl
Transformation of Compenent E. coli[Yale University]
% acetic acid (careful, only takes a few drops) Do not overshoot - it ruins the solution. You cannot adjust back with KOH. Sterilize by filtration Tfb II: 10mM MOPS 0.2g 75mM CaCl2*2H20 1.1g 10mM RbCl2 0.12g 15% glycerol(v/v) 15ml Adjust pH to 6
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