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文献和实验, followed by horizontal or vertical SDS electrophoresis (IPG-Dalt), was established in 1988 (2 ). Since that time, the protocol has not been changed essentially. Compared to classical 2-DE with carrier ampholytes (3 ,4 ), the employment of IPG-Dalt
IPG-Dalt of Very Alkaline Proteins
Compared to classical two-dimensional electrophoresis (2-DE) with carrier ampholytes (1 ,2 ), the advent of 2-DE with immobilized pH gradients (IPG-Dalt) (3 ) has produced significant improvements in 2-D electrophoretic separation
Immobilized pH gradient (IPG) as first dimension
Racket-shaped IPG A new sigmoidal immobilized pH gradient (IPG) was used as the first dimension. It offered high resolution and great reproducibility and allowed high protein loads. Based on our specifications, the non-linear pH gradient
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