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- 2025年07月20日
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文献和实验Labeling Tubulin with Tetramethylrhodamine Succinimidyl Ester
Materials 1.Drop frozen 2x cycled tubulin in a polymerization-promoting buffer.Stored at -80℃ 2.GTP,100 mM stock,titrate to pH ~7.Need 2 ml. 3.Waterbath,set at 37℃ 4.SS34 rotor,5 ml and 15 ml tubes and adaptors.Bring the rotor,adaptors,and tubes
LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
Day 0 and 1 Materials 1.0.5 mM ATP,0.2 mM CaCl2,1 mM PIPES,pH 6.8 at 4℃,250 ml for day 0. 2.0.5 mM ATP,0.2 mM CaCl2,70 mM KCl,150 mM PIPES,pH 7.0,1 ml. 3.16 mM ATP,0.2 mM CaCl2,5 mM DTT,5 mM lysine,10 mM glutamic acid,20 mM Tris-HCl,pH 8.0,10 ml
cross-linker, 1-pyrenebutanoic acid, succinimidyl ester. The technique effectively tethers the enzyme to the carbon nanotube which enhances bioelectrocatalysis, preserves the conductive nature of the carbon surface, and facilities direct electron
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