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文献和实验Porcine brain tubulin preparation
Homogenization buffer (freshly prepared) 1L PM buffer 100 mM MgATP 1L PMG Buffer 100 mM MgGTP 1M dithiothreitol (DTT) Glutamic acid sodium salt 实验设备 Waring Blender Temperature controlled ultracentrifuge
. In a 4oC cold room, carefully and thoroughly remove the meninges and any blood-red tissue from the surface, stem, and within the folds of each brain. Do this by both picking tissue away by hand and wiping with kimwipes. The dry wipe
Purification of Calmodulin from Bovine Brain
. Procedure (0-4℃): 1.About 1 kg of bovine brain (PelFreeze,frozen)is homogenized in 2 volumes of homogenizing buffer (50 mM MOPS,pH 7.0,1.0 mM 2-ME,1.0 mM EDTA)for 90 sec in a large Waring blender. 2.The homogenate is centrifuged at 10,000 x g for 1 hr
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