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- 2025年07月20日
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文献和实验Protocol for competitive RT-PCR
that containes a T7 RNA promoter sequence. The in vitro transcription of the cloned fragments is performed using T7 RNA polymerase (e.g. Gibco BRL). The internal standard RNA is then treated with RNase-free DNase I (e.g. Gibco BRL) to remove the plasmid DNA
DNA Preparation from Adherent Cells
Section of Cancer Genomics,Genetics Branch,NCI National Institutes of Health Reagents Chloroform Mallinckrodt,cat.4440 EDTA,0.5 M Ethanol,100% Ethanol,70% Isoamyl Alcohol Sigma,cat.I-3643 Phenol Gibco BRL,Cat.15513-039 Phosphate Buffered
You will need for 1° cDNA synthesis: 5 x SuperScript RTase buffer (Gibco-BRL) 5mM methyl dNTPs (Pharmacia, substitute 5m dCTP for dCTP) 100mM DTT (Gibco-BRL) oligo dT primer/adaptor [a-32P] dATP (~400Ci/mmol, Amersham or DuPont) Ribonuclease inhibitor
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