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- Cayman
- 30884-100 g
- 2025年07月20日
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文献和实验Methods for the preparation of Fly Extract (FE1 and FE2).
(1500 g) at 4° C for 15 mins. Switch on the 60°C waterbath. After the first spin the extract looks like this: 7. Remove supernatant, including surface fat/oil, from the spun tubes with a pasteur pipette
: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
lysis buffer (high salt) ) 2 x 1 ml CHIP wash buffer 2 x 1 ml TE elute immunoprecipitations: add 75 µl elution buffer incubate for 10 min at 65 °C spin, take supernatant, elute pellet again with 75 µl
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