- 询价
- ITI Biochem
- 美国
- 123334-13-4
- 2025年07月19日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
Phosphoenolpyruvic acid tris(cyclohexylammonium) salt
- 库存：
98
- 供应商：
北京孚博
- CAS号：
123334-13-4
- 规格：
1g
CAS：123334-13-4
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文献和实验in salt solutions containing 8 M urea, whereas the cell cytoplasm and the majority of nuclear components, except H3/H4, are washed away. This protocol yields highly purified H3/H4 in a few minutes without the use of chromatography steps. The acid
while retaining the oligo-dT bound poly（A）-RNA. After several washing steps under conditions less stringent than for annealing, poly（A）-RNA is eluted in water or low salt Tris buffer. Elution volumes vary from 20-250 μl for most kits accepting up to 1 mg of total
. High field strength (E) will not only increase the resolution, the experimental time is also reduced. Too high field strength may give heat problems if the cooling is inefficient, especially when focusing in the very basic or acid pH region
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