Diethyl (10-aminodecyl)phospho

Oligonucleoside Boranophosphate (Borane Phosphonate)

A number of short modified synthetic oligodeoxynucleotides have shown promise as agents for gene therapy ( 1 – 8 ). Among these, two classes of oligonucleotides with modified backbones (the phosphorothioates [ 9 ] and methylphosphonates [ 10

Oligodeoxynucleotide Synthesis: H-Phosphonate Approach

of laboratories have used acyl chlorides and nucleoside H-phosphonates in the synthesis of oligodeoxynucleotides (5 –9 ). This chemistry has also been extended to the synthesis of phosphate analogs of oligodeoxynucleotides (10 –16 ).

DIETHYL PYROCARBONATE (DEPC) TREATMENT OF AQUEOUS SOLUTIONS

Diethyl pyrocarbonate derivitizes histidine residues and is therefore an effective method to inactivate nucleases including RNAse. CAUTION: DEPC is irritating to the eyes, skin, and mucous membranes. It is a suspected carcinogen. It hydrolitically