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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
4-Bromo-5-chloro-8-methyl-2-phenylquinoline
- 库存:
98
- 供应商:
北京孚博
- CAS号:
1189106-24-8
- 规格:
1g
CAS:1189106-24-8
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文献和实验to replace combinattons of leucme coupled with valine in pepttde sequences. The preparation described is the final method developed, after many early less successful attempts. The synthesis mvolves (Fig. 1 ) the coupling of 1-bromo-5-methyl-2-hexene 3 and N
菌落。该产品筛选效果同蓝白斑筛选,且价格低廉。但需及时挑取白色菌落,当培养时间延长,白色菌落会逐渐变成微红色,影响挑选。 7、X-gal是5-溴-4-氯-3-吲哚-b-D-半乳糖(5-bromo-4-chloro-3-indolyl-b-D-galactoside)以半乳糖苷酶(b-galactosidase)水解后生成的吲哚衍生物显蓝色。IPTG是异丙基硫代半乳糖苷(Isopropylthiogalactoside),为非生理性的诱导物,它可以诱导lacZ的表达。 8、在含有
Transformation of Plasmids/Cosmids into E. Coli 质粒、粘粒转化大肠杆菌
of the chromogenic substrate 5-bromo-4-chloro-3-indolyl-beta-D-galacoside (X-gal). However insertion of a fragment of foreign DNA into the polycloning site of the plasmid almost invariably results in production of an amino-terminal fragment that is not capable
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