- 询价
- ITI Biochem
- 美国
- 1187386-22-6
- 2025年07月19日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
5-Bromo-1-t-butylbenzo[d]imidazole
- 库存：
98
- 供应商：
北京孚博
- CAS号：
1187386-22-6
- 规格：
1g
CAS：1187386-22-6
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文献和实验Protein Expression and Purification of MBP-T antigen (131-259)
-equilibrated benzamidine and Ni-NTA beads (set up gravity column) xvii.Collect FT and four washes of 25 ml using Buffer A containing 30 mM Imidazole.Run SDS-PAGE and select fraction containing T-ag (~15 kDa)and set-up dialysis against buffer CO/N and proceed
reached 1.0, isopropyl-1-thio-β-d-galactopyranoside was added to a final concentration of 1 mM. After 5 h of induction, the cells were harvested by centrifugation at 4,000 × g for 20 min. 样品处理 For the purification of protein from 2 liters of culture
contributed by Paul Brindley (1) Select "white/clear" plaques at random from lawns of phage-infected E. coli cultured on LB-agar containing 12.5 mg/ml tetracycline, 50 mg/ml 5-bromo-4-chloro-3-indoyl-D- galactopyranoside (X-Gal), and 50 mg/ml isopropyl-1-thio
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