(5-Fluorofuro[2,3-b]pyridin-2-

Convenient and Efficient Approach to the Permanent or Reversible Conjugation of RNA and DNA Sequences with Functional Groups

Figure 4.52.1 Synthesis of 2′‐ O ‐(aminooxymethyl)ribonucleosides (S.5 a‐d ). Abbreviations: BP , a uracil‐1‐yl, b N 4 ‐benzoylctytosin‐1‐yl, c N 6 ‐isobutyryladenin‐9‐yl, d N 2 ‐phenoxyacetylguanin‐9‐yl; DBU, 1,8‐diazabicyclo[5.4.0]undec‐7‐ene; B

Synthesis of Triazole‐Nucleoside Phosphoramidites and Their Use in Solid‐Phase Oligonucleotide Synthesis

Uracil (1 ), ≥99% pure Bromoacetic acid, 97% pure Methanol (MeOH), ACS grade

Biotinylation of a Propargylated Cyclic (3′‐5′) Diguanylic Acid and of Its Mono‐6‐Thioated Analog Under “Click” Conditions

) Methanol (MeOH, Fisher) 1H ‐Tetrazole (Glen Research) Anhydrous acetonitrile (MeCN, Glen Research