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- 保存条件：
Powder:2-8℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year
- 保质期：
Powder:2-8℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year
- 英文名：
3-Nitropropionic Acid
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- CAS号：
504-88-1
- 规格：
200mg/100mg
| 规格： | 200mg | 产品价格： | ￥880.0 |
|---|---|---|---|
| 规格： | 100mg | 产品价格： | ￥540.0 |
是琥珀酸脱氢酶的不可逆抑制剂。
【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】
标题:Ursolic acid alleviates meiotic abnormalities induced by 3-nitropropionic acid in mouse oocytes
成员:Tiancang Han, Zhaoyang Sun, Hongbo Zhang, Yuhan Zhao, Anhui Jiao, Qingshan Gao
论文因子:3.8 发表期刊:TOXICOLOGY AND APPLIED PHARMACOLOGY pmid:38521372
| 基本信息 | |
| CAS | No.504-88-1 |
| 中文名称 | 3-硝基PPA |
| 英文名称 | 3-Nitropropionic Acid |
| 别名 | β-Nitropropionic Acid;3-NP;;Bovinocidin |
| 分子式 | C3H5NO4 |
| 分子量 | 119.08 |
| 溶解性 | Soluble in Water/DMSO(Need ultrasonic) |
| 纯度 | ≥98% |
| 外观(性状) | Light yellow to orange Solid |
| 储存条件 | Powder:2-8℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year |
| EC | EINECS 208-003-0 |
| MDL | MFCD00007406 |
| SMILES | O=C(O)CC[N+]([O-])=O |
| InChIKey | WBLZUCOIBUDNBV-UHFFFAOYSA-N |
| InChI | InChI=1S/C3H5NO4/c5-3(6)1-2-4(7)8/h1-2H2,(H,5,6) |
| PubChem CID | 1678 |
| 靶点 | succinate dehydrogenase |
| 通路 | Metabolic Enzyme&Protease |
| 背景说明 | 3-Nitropropionic Acid是琥珀酸脱氢酶的不可逆抑制剂。 |
| 生物活性 | 3-Nitropropionic acid (β-Nitropropionic Acid, 3-NP) is an irreversible inhibitor of mitochondrial respiratory Complex II succinate dehydrogenase, resulting in energy depletion through disruption of the electron transport chain.[1-3] |
| In Vitro | 3-nitropropionic acid(3-NPA)is an irreversible inhibitor of complex II in the mitochondria. It impairs cellular energy metabolism via inhibition of succinate dehydrogenase，which induces a reduction in ATP production and leads to oxidative stress. 3-NPA can cause the generation and release of ROS from mitochondria，mitochondrial DNA damage，and thus loss of mitochondrial function. 3-NPA reduces the number of large follicles，impairs oocyte development，and increases the percentage of atretic large follicles and ROS levels in oocytes and granulosa cells[2]. ROS production followed by mitochondrial DNA damage is the primary event in 3-NPA toxicity，and Bcl-2 protects PC12 cells from 3-NPA toxicity by preventing mitochondrial DNA damage[3]. |
| 细胞实验 | Female ICR mice were dosed with 3-nitropropionic acid(3-NPA)at three different concentrations(6.25，12.5 and 25 mg/kg)and saline(control)via continuous intraperitoneal injection for 7 days. The treatment with 12.5 mg/kg reduced the weight of mouse ovaries，and significantly increased ROS levels and the activities of antioxidant enzymes—total superoxide dismutase(T-SOD)，glutathione peroxidase(GPx)and catalase(CAT)— in granulosa cells and ovarian tissues，but not in other tissues(brain，liver，kidney and spleen). The same treatment significantly increased the percentage of atretic large follicles，and reduced the number of large follicles，the number of ovulated oocytes，and the capacity for early embryonic development compared with controls. It also significantly decreased the ratio of Bcl-2 to Bax，while causing an increase in the mRNA expression of(SOD2，CAT and GPX)and ROS levels in granulosa cells. Collectively，these data indicate that 3-NPA induces granulosa cell apoptosis，large follicle atresia，and an increase of ROS levels in the ovary. [2] |
| 细胞实验 | Female，4-week-old ICR mice were kept at a constant temperature(22–24uC)in a 12 hr light/dark cycle with unrestricted access to food and water. For injection，3-NPA was dissolved in normal saline and the pH was adjusted to 7.4 with sodium hydroxide. Mice were randomly divided into four treatment groups(n = 20):(i)injected with normal saline solution(control);(ii)treated with 6.25 mg/kg 3-NPA;(iii)treated with 12.5 mg/kg 3-NPA;(iv)treated with 25 mg/kg 3-NPA; The control and experimental groups were injected intraperitoneally with a 0.1 ml dose of the appropriate solution twice daily，at 12 h intervals(8:00 a.m. and 8:00 p.m.)for 7 days. Weight gain，body and organ weights of mice treated with different doses of 3-NPA(6.25，12.5 and 25 mg/kg)and control were measured at 8 days.[2] |
| 动物实验 | Viability of the PC12 cells with or without treatment of 3-NPA is estimated using trypan blue exclusion test. Cells are grown in Petri dishes to a density of 1 × 106 cells/dish. After treatment with 4 or 8 mM 3-NPA for 48 h，cells are washed with Hank's balanced salt solution. Trypan blue(0.4 %)is added to the cells and incubated for 5 min. Both viable and nonviable cells are scored on a hemocytometer using a Nikon microscope. The nonviable cells are expressed as a percentage of total cell population[3] |
| 激酶实验 | After 3-NPA(12.5 mg/kg)treatment for seven days，different tissues(ovary，brain，spleen，liver and kidney)were collected. Separately，tissue from each of the five sampled organs(ovary，brain，spleen，liver and kidney)was homogenized in cold saline to prepare for the assay for activity of antioxidant enzymes.[2] |
| 数据来源文献 | [1] Huang LS， et al. J Biol Chem. 2006， 281(9):5965-72. [2] Zhang JQ， et al. PLoS One. 2014， 9(2):e86589. [3] Mandavilli BS， et al. Brain Res Mol Brain Res. 2005， 133(2):2 |
| 单位 | 瓶 |
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文献和实验【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】
标题:Ursolic acid alleviates meiotic abnormalities induced by 3-nitropropionic acid in mouse oocytes
成员:Tiancang Han, Zhaoyang Sun, Hongbo Zhang, Yuhan Zhao, Anhui Jiao, Qingshan Gao
论文因子:3.8 发表期刊:TOXICOLOGY AND APPLIED PHARMACOLOGY pmid:38521372
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包含天冬氨酸或天冬酰氨，谷氨酸或谷氨酰胺。 对-硝基苯基甲酮肟聚合物最早被DeGrado和Kaiser作为固相载体用于多肽的固相合成。肽基肟酯在酸性条件下稳定，但在氨解的条件下很不稳定。利用肟酯能够氨解的特点，Ospay等在合成环十肽Tyrocidine A（TA）时应用此法在肟树脂上合成直链的十肽，N端经TFA脱去Boc基后用DIEA中和，使氨基游离，室温搅拌24小时后，得到侧链保护的环肽，脱去保护基，纯化，得到收率高达55%的TA。 4. 酶法合成环肽 在缓冲液中利用蛋白酶
（1）石蜡切片脱蜡入水后，置0.1mol/l PBS pH7.2冲洗5min；冰冻切片直接入PBS冲洗5min。 （2）0.1mol/l 甘氨酸PBS冲洗5min。 （3）0.4%Trition X-100 PBS 冲洗15min。 （4）蛋白酶K1μg/ml（0.1mol/l Tris �HCl pH8.0, 50mmol/L EDTA配）37℃保温30min。 （5）4%多聚甲醛PBS固定5min。 （6）0.1mol/l PBS冲洗2×
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