- 询价
- ITI Biochem
- 美国
- 103192-39-8
- 2025年07月18日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
Guanosine 5-diphosphate tris salt from Saccharomyces cerevisiae
- 库存：
98
- 供应商：
北京孚博
- CAS号：
103192-39-8
- 规格：
1g
CAS：103192-39-8
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文献和实验Sample preparation (analytical gels)
at 4000 rpm in 10 ml low salt washing sample buffer: KCl 3.0 mM, KH2PO4 1.5 mM, NaCl 68 mM, NaH2PO4 9.0 mM. The pellet was then resuspended in 600 μl of a buffer containing 10 mM Tris-HCl pH 8.0, 1.5 mM MgCl2, 10 mM KCl, 0.5 mM DTE, 0.5 mM Pefabloc SC
Sample preparation (analytical gels)
containing Urea (8 M),CHAPS (4 % w/v),Tris (40 mM),DTE (65 mM)and a trace of bromophenol blue.After centrifugation at 10000 g for 5 minutes,the whole final diluted E.coli sample was loaded onto the IPG gel strip. YEAST (Saccharomyces cerevisiae): Cells
Sample preparation (preparative gels)
at 4° C and the pellet was washed 4 times for 10 min at 4000 rpm in 10 ml low salt washing sample buffer: KCl 3.0 mM, KH2PO4 1.5 mM, NaCl 68 mM, NaH2PO4 9.0 mM. The pellet was then resuspended in 600 µl of a buffer containing 10 mM Tris-HCl pH
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