- 询价
- ITI Biochem
- 美国
- 68938-01-2
- 2025年07月18日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
Deoxyribonucleic acids,salmon sperm
- 库存：
98
- 供应商：
北京孚博
- CAS号：
68938-01-2
- 规格：
1g
CAS：68938-01-2
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文献和实验Preparation of Salmon Sperm DNA
Denatured Salmon Sperm DNA is used in prehybridization and hybridization solutions to reduce background signal.The Salmon Sperm DNA (Sigma No.D-1626)is first "denatured" in large volumes and then again immediately before it is used.Two methods
Preparation of Sonicated Salmon Sperm DNA
Procedure: 1) Use Pharmacia #27-4564-01. With clean flamed scissors and forceps, weigh 0.25 g/50 ml conical and add 50 ml/conical of 0.02 M Tris, pH 7.6. Allow to dissolve over several days at 4°C
Salmon Sperm DNA1.Dissolve 1 g salmon sperm DNA in 100 ml H2 O. 2.Autoclave (20 minutes) and aliquot in 1 ml/tube 3.Store at -20℃. (common freezer for stock solutions)
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