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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
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- 保质期:
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- 英文名:
7-Bromo-4-chloro-5H-pyrrolo-[3,2-d]pyrimidine
- 库存:
98
- 供应商:
北京孚博
- CAS号:
1032650-41-1
- 规格:
1g
CAS:1032650-41-1
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文献和实验‐1) 4‐Chloro‐5‐iodo‐7H ‐pyrrolo[2,3‐d ]pyrimidine ( S.7 ; see Pudlo et al., ) 2‐Deoxy‐3,5‐di‐O ‐(p ‐toluoyl)‐α‐D‐erythro ‐pentofuranosyl chloride ( S.8 ; see Hoffer
in conjunction with streptavidin are horseradish peroxidase (HRP) or alkaline phosphate. These produce visible products over a period of 4 h in the presence of suitable substrates, e.g. 5-bromo-4-chloro-3-indolyl phosphate (BCIP) and nitro blue tetrazolium (NBT
1/2 volume of 1-bromo-3-chloro-propane (molecular biology grade, SIGMA) as chloroform substitute. For efficient recovery of small RNAs, DNA LoBind tubes (Eppendorf) were used and all centrifugation steps were performed at maximum speed and 4°C in an Eppendorf 5417R
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