7-Bromo-4-chloro-5H-pyrrolo-[3

Synthesis and Properties of 7‐Substituted 7‐Deazapurine (Pyrrolo[2,3‐d]pyrimidine) 2′‐Deoxyribonucleosides

‐1) 4‐Chloro‐5‐iodo‐7H ‐pyrrolo[2,3‐d ]pyrimidine ( S.7 ; see Pudlo et al., ) 2‐Deoxy‐3,5‐di‐O ‐(p ‐toluoyl)‐α‐D‐erythro ‐pentofuranosyl chloride ( S.8 ; see Hoffer

一篇整理的比较好的关于核酸定量的文章

in conjunction with streptavidin are horseradish peroxidase (HRP) or alkaline phosphate. These produce visible products over a period of 4 h in the presence of suitable substrates, e.g. 5-bromo-4-chloro-3-indolyl phosphate (BCIP) and nitro blue tetrazolium (NBT

MicroRNA Expression Profiling by Bead Array Technology in Human Tumor Cell Lines Treated with Interferon-Alpha-2a

1/2 volume of 1-bromo-3-chloro-propane (molecular biology grade, SIGMA) as chloroform substitute. For efficient recovery of small RNAs, DNA LoBind tubes (Eppendorf) were used and all centrifugation steps were performed at maximum speed and 4°C in an Eppendorf 5417R