6-(N-FMOC-PIPERAZIN-1-YL)-4(3H

Cell Signaling and Neurotoxicity: 3H-Arachidonic Acid Release (Phospholipase A2) in Cerebellar Granule Neurons

is arachidonic acid (AA). AA is abundant in the membrane phospholipids of the brain, where its release has been shown to be involved in synaptic plasticity processes, such as long-term potentiation. AA release is primarily produced by the activation

Amino acid composition

) to check derivatisation and chromatography efficiency and to allow quantitation of samples. Note that the Fmoc-amino acid derivatives are stable for 24 h, allowing many samples to be prepared in advance and loaded onto an autosampler for injection

Phosphoinositidase C Activation Assay I: Cell Labeling, Stimulation, and Recovery of Cellular [3H]Phosphoinositides and [3H]Phosphoinositols

. Some of the additional pathways that may be activated are shown by broken arrows. Abbreviations: PtdIns, phosphatidylinositol; PtdIns4P, phosphatidylinositol 4-phosphate; PtdIns(4,5)P 2 , phosphatidylinositol 4,5-bisphosphate; DG, diacylglycerol; PtdOH, phosphatidic acid